Publications

The LexA–RecA* structure reveals a cryptic lock-and-key mechanism for SOS activation

Highly tunable bimane-based fluorescent probes: design, synthesis, and application as a selective amyloid binding dye

Long-distance tmFRET using bipyridyl- and phenanthroline-based ligands

Measuring conformational equilibria in allosteric proteins with time-resolved tmFRET

Global analysis of post-translational sidechain arginylation using pan-arginylation antibodies

The Effects of Lipids on α-Synuclein Aggregation In Vitro

Combining non-canonical amino acid mutagenesis and native chemical ligation for multiply modifying proteins: A case study of α-synuclein post-translational modifications

A Novel Brain PET Radiotracer for Imaging Alpha Synuclein Fibrils in Multiple System Atrophy

AggBERT: Best in Class Prediction of Hexapeptide Amyloidogenesis with a Semi-Supervised ProtBERT Model

Semi-Synthetic CoA-α-Synuclein Constructs Trap N-Terminal Acetyltransferase NatB for Binding Mechanism Studies

Synthesis of Peptides and Proteins with Site-Specific Glutamate Arginylation

FRETing about the details: Case studies in the use of a genetically encoded fluorescent amino acid for distance‐dependent energy transfer

Identification of a Putative α-synuclein Radioligand Using an in silico Similarity Search

Computational Chemistry for the Identification of Lead Compounds for Radiotracer Development

Post-translational modifications of soluble α-synuclein regulate the amplification of pathological α-synuclein

Molecular role of NAA38 in thermostability and catalytic activity of the human NatC N-terminal acetyltransferase

Engineered RecA Constructs Reveal the Minimal SOS Activation Complex

Generating Efficient Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetases for Structurally Diverse Non-Canonical Amino Acids

Effects of Mutations and Post-Translational Modifications on α-Synuclein In Vitro Aggregation

Harnessing the intrinsic photochemistry of isoxazoles for the development of chemoproteomic crosslinking methods

A Bond-Energy/Bond-Order and Populations Relationship

Cysteine-Based Mimic of Arginylation Reproduces Neuroprotective Effects of the Authentic Post-Translational Modification on α-Synuclein

α-Synuclein arginylation in the human brain

Structural impact of thioamide incorporation into a β-hairpin

Alpha-synuclein from patient Lewy bodies exhibits distinct pathological activity that can be propagated in vitro

An improved fluorescent noncanonical amino acid for measuring conformational distributions using time-resolved transition metal ion FRET

Biomolecular simulation based machine learning models accurately predict sites of tolerability to the unnatural amino acid acridonylalanine

Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells

Rational design of thioamide peptides as selective inhibitors of cysteine protease cathepsin L

New strategies for fluorescently labeling proteins in the study of amyloids

Molecular mechanism of N-terminal acetylation by the ternary NatC complex

Incorporating thioamides into proteins by native chemical ligation

Chemoenzymatic Semi‐synthesis Enables Efficient Production of Isotopically‐Labelled α‐Synuclein with Site‐specific Tyrosine Phosphorylation

Synthesis and characterization of fluorescent amino acid dimethylaminoacridonylalanine

Somatostatin Receptor as a Molecular Imaging Target in Human and Canine Cushing Disease

Side‐chain thioamides as fluorescence quenching probes

Insights into genome recoding from the mechanism of a classic +1-frameshifting tRNA

Effects of Glutamate Arginylation on α-Synuclein: Studying an Unusual Post-Translational Modification through Semisynthesis

Improved Modeling of Thioamide FRET Quenching by Including Conformational Restriction and Coulomb Coupling

Identification of a nanomolar affinity α-synuclein fibril imaging probe by ultra-high throughput in silico screening

Molecular basis for N-terminal alpha-synuclein acetylation by human NatB

Rosetta Machine Learning Models Accurately Classify Positional Effects of Thioamides on Proteolysis

Rational design of small molecule fluorescent probes for biological applications

Quinoline-based fluorescent small molecules for live cell imaging

Evaluation of Diagnostic Accuracy Following the Coadministration of Delta-Aminolevulinic Acid and Second Window Indocyanine Green in Rodent and Human Glioblastomas

A Unified De Novo Approach for Predicting the Structures of Ordered and Disordered Proteins

Rosetta custom score functions accurately predict ΔΔG of mutations at protein–protein interfaces using machine learning

Protein labeling for FRET with methoxycoumarin and acridonylalanine

Studies of Thioamide Effects on Serine Protease Activity Enable Two-Site Stabilization of Cancer Imaging Peptides

Synthesis and characterization of high affinity fluorogenic α-synuclein probes

Chemoenzymatic Semisynthesis of Phosphorylated α-Synuclein Enables Identification of a Bidirectional Effect on Fibril Formation

The Kinetic and Molecular Basis for the Interaction of LexA and Activated RecA Revealed by a Fluorescent Amino Acid Probe

Thioamide-Containing Peptides and Proteins

Fluorescent Probes for Studying Thioamide Positional Effects on Proteolysis Reveal Insight into Resistance to Cysteine Proteases

Structure and Mechanism of Acetylation by the N-Terminal Dual Enzyme NatA/Naa50 Complex

Expanding Genetic Code Expansion through Resource Facilities, Workshops, and Conferences

Cyclized NDGA modifies dynamic α-synuclein monomers preventing aggregation and toxicity

Biosynthesis and Chemical Applications of Thioamides

A “Clickable” Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

A PARP-1 Feed-Forward Mechanism To Accelerate α-Synuclein Toxicity in Parkinson’s Disease

Optimization of Second Window Indocyanine Green for Intraoperative Near-Infrared Imaging of Thoracic Malignancy

Systematic Evaluation of Soluble Protein Expression Using a Fluorescent Unnatural Amino Acid Reveals No Reliable Predictors of Tolerability

Rational Design and Facile Synthesis of a Highly Tunable Quinoline-Based Fluorescent Small-Molecule Scaffold for Live Cell Imaging

Alpha Synuclein Fibrils Contain Multiple Binding Sites for Small Molecules

Improving the fluorescent probe acridonylalanine through a combination of theory and experiment

Dithioamide Substitutions in Proteins: Effects on thermostability, peptide binding, and fluorescence quenching in calmodulin

Using a FRET Library with Multiple Probe Pairs To Drive Monte Carlo Simulations of α-Synuclein

Scalable thioarylation of unprotected peptides and biomolecules under Ni/photoredox catalysis

Fluorescence spectroscopy reveals N-terminal order in fibrillar forms of α-synuclein

Thioamide Substitution Selectively Modulates Proteolysis and Receptor Activity of Therapeutic Peptide Hormones

A cryptophane-based “turn-on” 129Xe NMR biosensor for monitoring calmodulin

Multicolor Protein FRET with Tryptophan, Selective Coumarin-Cysteine Labeling, and Genetic Acridonylalanine Encoding

Selective imaging of internalized proteopathic α-synuclein seeds in primary neurons reveals mechanistic insight into transmission of synucleinopathies

Thioamide Labeling of Proteins through a Combination of Semisynthetic Methods

Thieme Chemistry Journals Awardees – Where Are They Now? Special Issue

Improved Fmoc Deprotection Methods for the Synthesis of Thioamide-Containing Peptides and Proteins

Featured in Synform!

Effect of nascent peptide steric bulk on elongation kinetics in the ribosome exit tunnel

Improving target amino acid selectivity in a permissive aminoacyl tRNA synthetase through counter-selection

Potential Artifacts in Sample Preparation Methods Used for Imaging Amyloid Oligomers and Protofibrils due to Surface-Mediated Fibril Formation

The Effects of Thioamide Backbone Substitution on Protein Stability: A study in α-helical, β-sheet, and polyproline II helical contexts

Site-Specific Fluorescence Polarization for Studying the Disaggregation of α-Synuclein Fibrils by Small Molecules

Chemoselective modifications for the traceless ligation of thioamide-containing peptides and proteins

Electronic interactions of i, i + 1 dithioamides: increased fluorescence quenching and evidence for n-to-π* interactions

Comparison of strategies for non-perturbing labeling of α-synuclein to study amyloidogenesis

Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles

Multiply labeling proteins for studies of folding and stability

Semi-synthesis of thioamide containing proteins

Inteins as Traceless Purification Tags for Unnatural Amino Acid Proteins

Specific Modulation of Protein Activity by Using a Bioorthogonal Reaction

Surface Effects Mediate Self-Assembly of Amyloid-β Peptides

Characterization of the Lipid Binding Properties of Otoferlin Reveal Specific Interactions Between PI(4,5)P2 and the C2C and C2F Domains

Two-for-one designer labels

On the use of thioamides as fluorescence quenching probes for tracking protein folding and stability

Thioamide-Based Fluorescent Protease Sensors

Minimalist Approaches to Protein Labelling: Getting the Most Fluorescent Bang for Your Steric Buck

Included in Aust. J. Chem. 2013 Physical Organic Chemistry Gordon Research Conference Issue.

Synthesis of thioester peptides for the incorporation of thioamides into proteins by native chemical ligation

Efficient Synthesis and In Vivo Incorporation of Acridon-2-ylalanine, a Fluorescent Amino Acid for Lifetime and Förster Resonance Energy Transfer/Luminescence Resonance Energy Transfer Studies

Thioamide Quenching of Fluorescent Probes through Photoinduced Electron Transfer: Mechanistic Studies and Applications

Efficient, Traceless Semi-Synthesis of α-Synuclein Labeled with a Fluorophore/Thioamide FRET Pair

Included in Synlett 2013 EuCheMS Young Investigator Workshop Issue.

Molecular basis for N-terminal acetylation by the heterodimeric NatA complex

Labeling Proteins with Fluorophore/Thioamide Förster Resonant Energy Transfer Pairs by Combining Unnatural Amino Acid Mutagenesis and Native Chemical Ligation

Expressed Protein Ligation at Methionine: N-Terminal Attachment of Homocysteine, Ligation, and Masking

Native Chemical Ligation of Thioamide-Containing Peptides: Development and Application to the Synthesis of Labeled α-Synuclein for Misfolding Studies

Highlighted in C&E News: Chem. Eng. News 90 34 (2012).

Spotlighted in JACS: J. Am. Chem. Soc. 134 6499 (2012).

Minimalist Probes for Studying Protein Dynamics: Thioamide Quenching of Selectively Excitable Fluorescent Amino Acids

Highlighted in C&E News: Chem. Eng. News 90 34 (2012).

Thioamide Quenching of Intrinsic Protein Fluorescence

N-Terminal Protein Modification Using Simple Aminoacyl Transferase Substrates

Thioamides as Fluorescence Quenching Probes: Minimalist Chromophores to Monitor Protein Dynamics